SB1aDOK 2
1) Which statement best compares the structure and function of ribosomes and mitochondria?
Ribosomes are the site of protein synthesis; mitochondria perform aerobic respiration to produce ATP.
SB1c (Enzymes)DOK 2
2) An enzyme’s active site is complementary to its substrate. Which change is most likely to decrease enzyme activity?
High temperature can denature enzymes, altering the active site so the substrate no longer binds effectively.
SB1b (Macromolecules)DOK 2
3) Which pairing correctly matches a macromolecule with its primary function?
Carbohydrates provide quick energy (glucose) and structural roles (cellulose in plants, chitin in arthropods).
SB1a/SB1d (Membranes)DOK 2
4) A cell placed in a hypertonic solution will most likely:
Hypertonic surroundings draw water out of the cell via osmosis, causing it to shrink (plasmolysis in plants).
SB1c (Enzyme graphs)DOK 3
5) A student measures the rate of catalase at different pH values (data below). Which conclusion is best supported?
pH467810
Relative rate0.30.81.00.70.2
The peak rate (1.0) at pH 7 indicates an optimum near neutral with decreased activity at more acidic/basic pH.
SB1d (Transport/energy)DOK 3
6) A cell must accumulate iodide ions to a concentration 10× higher than outside. Which transport mechanism is most likely used, and why?
Moving solutes from low to high concentration requires energy; membrane pumps use ATP for active transport.
SB1a (SA:V & cells)DOK 3
7) Small cells exchange materials more efficiently than large cells. Which reasoning best explains this?
As size increases, volume grows faster than surface area; small cells maintain a high SA:V for faster exchange per unit volume.
SB1b/SB1c (Macromolecules & enzymes)DOK 3
8) A food lab tests three unknowns. Results: (I) positive Benedict’s (reducing sugars), (II) positive Biuret (peptide bonds), (III) hydrophobic layer separates in water. Which claim is most supported?
Benedict’s → reducing sugars (carbohydrates); Biuret → proteins; hydrophobic/separates → lipids.
SB1d (Homeostasis & transport)Constructed Response
9) A plant cell is transferred from a hypotonic environment to a hypertonic environment. Explain how water movement changes and predict two cellular effects you would observe after 30 minutes. Connect your explanation to membrane structure and osmosis.
2-point Rubric:
  • 2 pts: Correctly describes water moving out in hypertonic conditions (osmosis), links to phospholipid bilayer/selective permeability, and predicts two valid effects (e.g., plasmolysis, decreased turgor, membrane pull from cell wall, wilting).
  • 1 pt: Partially correct description (one valid effect or vague mechanism).
  • 0 pts: Incorrect or irrelevant.
Show exemplar response
In the hypertonic solution, water exits the plant cell by osmosis across the selectively permeable phospholipid bilayer. As water leaves, turgor pressure drops and the membrane pulls away from the cell wall (plasmolysis). After ~30 minutes the cell appears flaccid/wilted and metabolic rates may slow due to loss of water balance.
SB1c (Enzymes & conditions)Constructed Response
10) A bakery wants its amylase to break starch into glucose rapidly at room temperature. Recommend two changes to maximize reaction rate and justify them using enzyme principles. Include a potential risk if each change is overapplied.
2-point Rubric:
  • 2 pts: Two correct strategies with reasoning (e.g., increase enzyme or substrate concentration up to saturation; adjust pH toward optimum; maintain moderate temperature near optimum) and a valid overapplication risk (e.g., denaturation at high temp or extreme pH; diminishing returns at saturation).
  • 1 pt: One correct strategy with partial justification, limited or missing risks.
  • 0 pts: Incorrect recommendations or no justification.
Show exemplar response
Increase enzyme concentration so more active sites are available, which raises rate until substrate becomes limiting (risk: waste/cost once saturated). Adjust pH toward amylase’s optimum to maximize active-site fit (risk: extreme pH can denature and reduce activity).
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